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ecotropic packaging helper plasmid pcl eco  (Addgene inc)


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    Structured Review

    Addgene inc ecotropic packaging helper plasmid pcl eco
    Ecotropic Packaging Helper Plasmid Pcl Eco, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecotropic+packaging+plasmid+pcl+eco/pCL-Eco+(Plasmid+%2312371)/pmc13013783-127-11-16
    Average 96 stars, based on 458 article reviews
    ecotropic packaging helper plasmid pcl eco - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Retroviral:

    Article Title: Telomeres acquire embryonic stem cell characteristics in induced pluripotent stem cells.
    Article Snippet: .. Briefly, retroviral supernatants were produced in HEK293T cells (5 3 106 cells per 100 mm diameter dish) transfected with the ecotropic packaging plasmid pCL-Eco (4 mg) together with either one of the following retroviral constructs (4 mg): pMXs-cMyc, pMXs-Klf4, pMXs-Sox2, or pMXs-Oct3/4 (Addgene). .. Transfections were performed using Fugene-6 transfection reagent (Roche) according to the manufacturer’s protocol.

    Article Title: The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity
    Article Snippet: .. For retrovirus, per dish, 293T cells were transfected with the ecotropic packaging plasmid pCL-Eco (4 μg) together with one of the following retroviral constructs (4 μg): pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, pMXs-cMyc, or pMXs-Nanog (obtained from Addgene and previously described (Takahashi and Yamanaka, 2006) -the backbone is pMXs plasmid in all cases and the expression of the coding sequences of the reprogramming factors are driven by the MMLV LTR promoter. ..

    Article Title: Modulation of telomere protection by the PI3K/AKT pathway.
    Article Snippet: .. Retroviral supernatants were produced in 293T cells (5 × 105 cells per 100 mm diameter dish) transfected with the ecotropic packaging plasmid pCL-Eco and either pBabe-Cre, pBabe-GFP or eGFP-TRF1 pWzl-Hygro (a gift from T. de Lange, Addgene plasmid #19834). ..

    Article Title: Activation of AMP-activated protein kinase (AMPK) provides a metabolic barrier to reprogramming somatic cells into stem cells.
    Article Snippet: .. The reprogramming of primary (passage 2–4) mouse embryo fibroblasts (MEFs) derived from wild-type embryos (C57BL6 genetic background) was performed as previously described by Marion et al.17 with modifications from a previous protocol by Blelloch et al.136 Briefly, retroviral supernatants were produced in HEK-293T cells (ATCC® Number CRL-11268TM) transfected with 4 μg of the ecotropic packaging plasmid pCL-Eco (www.addgene. org/12371/) together with 4 μg of one of the following retroviral constructs: pMX-Oct3/4 (www.addgene.org/13366/), pMXSox2 (www.addgene.org/13367/) or pMX-Klf4 (www.addgene. org/13370/). .. Transfections were performed using the PlusTM Reagent (Invitrogen) according to the manufacturer’s protocol.

    Article Title: Metformin lowers the threshold for stress-induced senescence: a role for the microRNA-200 family and miR-205.
    Article Snippet: .. Briefly, retroviral supernatants were produced in HEK-293T cells (ATCC, CRL11268TM) transfected with 4 μg of the ecotropic packaging plasmid pCL-Eco (www.addgene.org/12371/) together with 4 μg of one of the following retroviral constructs: pMX-OCT3/4 (www. addgene.org/13366/), pMX-SOX2 (www.addgene.org/13367/) or pMX-KLF4 (www.addgene.org/13370/). .. Transfections were performed using the PlusTM Reagent (Invitrogen) according to the manufacturer’s protocol.

    Article Title: The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity
    Article Snippet: .. For retrovirus, per dish, 293T cells were transfected with the ecotropic packaging plasmid pCL-Eco (4 μg) together with one of the following retroviral constructs (4 μg): pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, pMXs-cMyc, or pMXs-Nanog (obtained from Addgene and previously described (Takahashi and Yamanaka, 2006) -the backbone is pMXs plasmid in all cases and the expression of the coding sequences of the reprogramming factors are driven by the MMLV LTR promoter. ..

    Produced:

    Article Title: Telomeres acquire embryonic stem cell characteristics in induced pluripotent stem cells.
    Article Snippet: .. Briefly, retroviral supernatants were produced in HEK293T cells (5 3 106 cells per 100 mm diameter dish) transfected with the ecotropic packaging plasmid pCL-Eco (4 mg) together with either one of the following retroviral constructs (4 mg): pMXs-cMyc, pMXs-Klf4, pMXs-Sox2, or pMXs-Oct3/4 (Addgene). .. Transfections were performed using Fugene-6 transfection reagent (Roche) according to the manufacturer’s protocol.

    Article Title: Modulation of telomere protection by the PI3K/AKT pathway.
    Article Snippet: .. Retroviral supernatants were produced in 293T cells (5 × 105 cells per 100 mm diameter dish) transfected with the ecotropic packaging plasmid pCL-Eco and either pBabe-Cre, pBabe-GFP or eGFP-TRF1 pWzl-Hygro (a gift from T. de Lange, Addgene plasmid #19834). ..

    Article Title: Activation of AMP-activated protein kinase (AMPK) provides a metabolic barrier to reprogramming somatic cells into stem cells.
    Article Snippet: .. The reprogramming of primary (passage 2–4) mouse embryo fibroblasts (MEFs) derived from wild-type embryos (C57BL6 genetic background) was performed as previously described by Marion et al.17 with modifications from a previous protocol by Blelloch et al.136 Briefly, retroviral supernatants were produced in HEK-293T cells (ATCC® Number CRL-11268TM) transfected with 4 μg of the ecotropic packaging plasmid pCL-Eco (www.addgene. org/12371/) together with 4 μg of one of the following retroviral constructs: pMX-Oct3/4 (www.addgene.org/13366/), pMXSox2 (www.addgene.org/13367/) or pMX-Klf4 (www.addgene. org/13370/). .. Transfections were performed using the PlusTM Reagent (Invitrogen) according to the manufacturer’s protocol.

    Article Title: Metformin lowers the threshold for stress-induced senescence: a role for the microRNA-200 family and miR-205.
    Article Snippet: .. Briefly, retroviral supernatants were produced in HEK-293T cells (ATCC, CRL11268TM) transfected with 4 μg of the ecotropic packaging plasmid pCL-Eco (www.addgene.org/12371/) together with 4 μg of one of the following retroviral constructs: pMX-OCT3/4 (www. addgene.org/13366/), pMX-SOX2 (www.addgene.org/13367/) or pMX-KLF4 (www.addgene.org/13370/). .. Transfections were performed using the PlusTM Reagent (Invitrogen) according to the manufacturer’s protocol.

    Transfection:

    Article Title: Telomeres acquire embryonic stem cell characteristics in induced pluripotent stem cells.
    Article Snippet: .. Briefly, retroviral supernatants were produced in HEK293T cells (5 3 106 cells per 100 mm diameter dish) transfected with the ecotropic packaging plasmid pCL-Eco (4 mg) together with either one of the following retroviral constructs (4 mg): pMXs-cMyc, pMXs-Klf4, pMXs-Sox2, or pMXs-Oct3/4 (Addgene). .. Transfections were performed using Fugene-6 transfection reagent (Roche) according to the manufacturer’s protocol.

    Article Title: The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity
    Article Snippet: .. For retrovirus, per dish, 293T cells were transfected with the ecotropic packaging plasmid pCL-Eco (4 μg) together with one of the following retroviral constructs (4 μg): pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, pMXs-cMyc, or pMXs-Nanog (obtained from Addgene and previously described (Takahashi and Yamanaka, 2006) -the backbone is pMXs plasmid in all cases and the expression of the coding sequences of the reprogramming factors are driven by the MMLV LTR promoter. ..

    Article Title: Modulation of telomere protection by the PI3K/AKT pathway.
    Article Snippet: .. Retroviral supernatants were produced in 293T cells (5 × 105 cells per 100 mm diameter dish) transfected with the ecotropic packaging plasmid pCL-Eco and either pBabe-Cre, pBabe-GFP or eGFP-TRF1 pWzl-Hygro (a gift from T. de Lange, Addgene plasmid #19834). ..

    Article Title: Activation of AMP-activated protein kinase (AMPK) provides a metabolic barrier to reprogramming somatic cells into stem cells.
    Article Snippet: .. The reprogramming of primary (passage 2–4) mouse embryo fibroblasts (MEFs) derived from wild-type embryos (C57BL6 genetic background) was performed as previously described by Marion et al.17 with modifications from a previous protocol by Blelloch et al.136 Briefly, retroviral supernatants were produced in HEK-293T cells (ATCC® Number CRL-11268TM) transfected with 4 μg of the ecotropic packaging plasmid pCL-Eco (www.addgene. org/12371/) together with 4 μg of one of the following retroviral constructs: pMX-Oct3/4 (www.addgene.org/13366/), pMXSox2 (www.addgene.org/13367/) or pMX-Klf4 (www.addgene. org/13370/). .. Transfections were performed using the PlusTM Reagent (Invitrogen) according to the manufacturer’s protocol.

    Article Title: Metformin lowers the threshold for stress-induced senescence: a role for the microRNA-200 family and miR-205.
    Article Snippet: .. Briefly, retroviral supernatants were produced in HEK-293T cells (ATCC, CRL11268TM) transfected with 4 μg of the ecotropic packaging plasmid pCL-Eco (www.addgene.org/12371/) together with 4 μg of one of the following retroviral constructs: pMX-OCT3/4 (www. addgene.org/13366/), pMX-SOX2 (www.addgene.org/13367/) or pMX-KLF4 (www.addgene.org/13370/). .. Transfections were performed using the PlusTM Reagent (Invitrogen) according to the manufacturer’s protocol.

    Article Title: The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity
    Article Snippet: .. For retrovirus, per dish, 293T cells were transfected with the ecotropic packaging plasmid pCL-Eco (4 μg) together with one of the following retroviral constructs (4 μg): pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, pMXs-cMyc, or pMXs-Nanog (obtained from Addgene and previously described (Takahashi and Yamanaka, 2006) -the backbone is pMXs plasmid in all cases and the expression of the coding sequences of the reprogramming factors are driven by the MMLV LTR promoter. ..

    Plasmid Preparation:

    Article Title: Telomeres acquire embryonic stem cell characteristics in induced pluripotent stem cells.
    Article Snippet: .. Briefly, retroviral supernatants were produced in HEK293T cells (5 3 106 cells per 100 mm diameter dish) transfected with the ecotropic packaging plasmid pCL-Eco (4 mg) together with either one of the following retroviral constructs (4 mg): pMXs-cMyc, pMXs-Klf4, pMXs-Sox2, or pMXs-Oct3/4 (Addgene). .. Transfections were performed using Fugene-6 transfection reagent (Roche) according to the manufacturer’s protocol.

    Article Title: The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity
    Article Snippet: .. For retrovirus, per dish, 293T cells were transfected with the ecotropic packaging plasmid pCL-Eco (4 μg) together with one of the following retroviral constructs (4 μg): pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, pMXs-cMyc, or pMXs-Nanog (obtained from Addgene and previously described (Takahashi and Yamanaka, 2006) -the backbone is pMXs plasmid in all cases and the expression of the coding sequences of the reprogramming factors are driven by the MMLV LTR promoter. ..

    Article Title: Acidity suppresses CD8 + T-cell function by perturbing IL-2, mTORC1, and c-Myc signaling
    Article Snippet: .. The ecotropic packaging plasmid pCL-Eco was a gift from Inder Verma (Addgene plasmid # 12371; http://n2t.net/addgene:12371 ; RRID: Addgene_12371 ) (Naviaux et al, ). .. 293T were transfected using the calcium phosphate technique (Jordan et al, ) with a 1:1 retroviral vector to packaging plasmid ratio.

    Article Title: Modulation of telomere protection by the PI3K/AKT pathway.
    Article Snippet: .. Retroviral supernatants were produced in 293T cells (5 × 105 cells per 100 mm diameter dish) transfected with the ecotropic packaging plasmid pCL-Eco and either pBabe-Cre, pBabe-GFP or eGFP-TRF1 pWzl-Hygro (a gift from T. de Lange, Addgene plasmid #19834). ..

    Article Title: Activation of AMP-activated protein kinase (AMPK) provides a metabolic barrier to reprogramming somatic cells into stem cells.
    Article Snippet: .. The reprogramming of primary (passage 2–4) mouse embryo fibroblasts (MEFs) derived from wild-type embryos (C57BL6 genetic background) was performed as previously described by Marion et al.17 with modifications from a previous protocol by Blelloch et al.136 Briefly, retroviral supernatants were produced in HEK-293T cells (ATCC® Number CRL-11268TM) transfected with 4 μg of the ecotropic packaging plasmid pCL-Eco (www.addgene. org/12371/) together with 4 μg of one of the following retroviral constructs: pMX-Oct3/4 (www.addgene.org/13366/), pMXSox2 (www.addgene.org/13367/) or pMX-Klf4 (www.addgene. org/13370/). .. Transfections were performed using the PlusTM Reagent (Invitrogen) according to the manufacturer’s protocol.

    Article Title: Metformin lowers the threshold for stress-induced senescence: a role for the microRNA-200 family and miR-205.
    Article Snippet: .. Briefly, retroviral supernatants were produced in HEK-293T cells (ATCC, CRL11268TM) transfected with 4 μg of the ecotropic packaging plasmid pCL-Eco (www.addgene.org/12371/) together with 4 μg of one of the following retroviral constructs: pMX-OCT3/4 (www. addgene.org/13366/), pMX-SOX2 (www.addgene.org/13367/) or pMX-KLF4 (www.addgene.org/13370/). .. Transfections were performed using the PlusTM Reagent (Invitrogen) according to the manufacturer’s protocol.

    Article Title: The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity
    Article Snippet: .. For retrovirus, per dish, 293T cells were transfected with the ecotropic packaging plasmid pCL-Eco (4 μg) together with one of the following retroviral constructs (4 μg): pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, pMXs-cMyc, or pMXs-Nanog (obtained from Addgene and previously described (Takahashi and Yamanaka, 2006) -the backbone is pMXs plasmid in all cases and the expression of the coding sequences of the reprogramming factors are driven by the MMLV LTR promoter. ..

    Construct:

    Article Title: Telomeres acquire embryonic stem cell characteristics in induced pluripotent stem cells.
    Article Snippet: .. Briefly, retroviral supernatants were produced in HEK293T cells (5 3 106 cells per 100 mm diameter dish) transfected with the ecotropic packaging plasmid pCL-Eco (4 mg) together with either one of the following retroviral constructs (4 mg): pMXs-cMyc, pMXs-Klf4, pMXs-Sox2, or pMXs-Oct3/4 (Addgene). .. Transfections were performed using Fugene-6 transfection reagent (Roche) according to the manufacturer’s protocol.

    Article Title: The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity
    Article Snippet: .. For retrovirus, per dish, 293T cells were transfected with the ecotropic packaging plasmid pCL-Eco (4 μg) together with one of the following retroviral constructs (4 μg): pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, pMXs-cMyc, or pMXs-Nanog (obtained from Addgene and previously described (Takahashi and Yamanaka, 2006) -the backbone is pMXs plasmid in all cases and the expression of the coding sequences of the reprogramming factors are driven by the MMLV LTR promoter. ..

    Article Title: Activation of AMP-activated protein kinase (AMPK) provides a metabolic barrier to reprogramming somatic cells into stem cells.
    Article Snippet: .. The reprogramming of primary (passage 2–4) mouse embryo fibroblasts (MEFs) derived from wild-type embryos (C57BL6 genetic background) was performed as previously described by Marion et al.17 with modifications from a previous protocol by Blelloch et al.136 Briefly, retroviral supernatants were produced in HEK-293T cells (ATCC® Number CRL-11268TM) transfected with 4 μg of the ecotropic packaging plasmid pCL-Eco (www.addgene. org/12371/) together with 4 μg of one of the following retroviral constructs: pMX-Oct3/4 (www.addgene.org/13366/), pMXSox2 (www.addgene.org/13367/) or pMX-Klf4 (www.addgene. org/13370/). .. Transfections were performed using the PlusTM Reagent (Invitrogen) according to the manufacturer’s protocol.

    Article Title: Metformin lowers the threshold for stress-induced senescence: a role for the microRNA-200 family and miR-205.
    Article Snippet: .. Briefly, retroviral supernatants were produced in HEK-293T cells (ATCC, CRL11268TM) transfected with 4 μg of the ecotropic packaging plasmid pCL-Eco (www.addgene.org/12371/) together with 4 μg of one of the following retroviral constructs: pMX-OCT3/4 (www. addgene.org/13366/), pMX-SOX2 (www.addgene.org/13367/) or pMX-KLF4 (www.addgene.org/13370/). .. Transfections were performed using the PlusTM Reagent (Invitrogen) according to the manufacturer’s protocol.

    Article Title: The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity
    Article Snippet: .. For retrovirus, per dish, 293T cells were transfected with the ecotropic packaging plasmid pCL-Eco (4 μg) together with one of the following retroviral constructs (4 μg): pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, pMXs-cMyc, or pMXs-Nanog (obtained from Addgene and previously described (Takahashi and Yamanaka, 2006) -the backbone is pMXs plasmid in all cases and the expression of the coding sequences of the reprogramming factors are driven by the MMLV LTR promoter. ..

    Expressing:

    Article Title: The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity
    Article Snippet: .. For retrovirus, per dish, 293T cells were transfected with the ecotropic packaging plasmid pCL-Eco (4 μg) together with one of the following retroviral constructs (4 μg): pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, pMXs-cMyc, or pMXs-Nanog (obtained from Addgene and previously described (Takahashi and Yamanaka, 2006) -the backbone is pMXs plasmid in all cases and the expression of the coding sequences of the reprogramming factors are driven by the MMLV LTR promoter. ..

    Article Title: The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity
    Article Snippet: .. For retrovirus, per dish, 293T cells were transfected with the ecotropic packaging plasmid pCL-Eco (4 μg) together with one of the following retroviral constructs (4 μg): pMXs-Oct4, pMXs-Sox2, pMXs-Klf4, pMXs-cMyc, or pMXs-Nanog (obtained from Addgene and previously described (Takahashi and Yamanaka, 2006) -the backbone is pMXs plasmid in all cases and the expression of the coding sequences of the reprogramming factors are driven by the MMLV LTR promoter. ..

    Derivative Assay:

    Article Title: Activation of AMP-activated protein kinase (AMPK) provides a metabolic barrier to reprogramming somatic cells into stem cells.
    Article Snippet: .. The reprogramming of primary (passage 2–4) mouse embryo fibroblasts (MEFs) derived from wild-type embryos (C57BL6 genetic background) was performed as previously described by Marion et al.17 with modifications from a previous protocol by Blelloch et al.136 Briefly, retroviral supernatants were produced in HEK-293T cells (ATCC® Number CRL-11268TM) transfected with 4 μg of the ecotropic packaging plasmid pCL-Eco (www.addgene. org/12371/) together with 4 μg of one of the following retroviral constructs: pMX-Oct3/4 (www.addgene.org/13366/), pMXSox2 (www.addgene.org/13367/) or pMX-Klf4 (www.addgene. org/13370/). .. Transfections were performed using the PlusTM Reagent (Invitrogen) according to the manufacturer’s protocol.



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